全文获取类型
收费全文 | 16452篇 |
免费 | 1884篇 |
国内免费 | 1177篇 |
专业分类
耳鼻咽喉 | 134篇 |
儿科学 | 399篇 |
妇产科学 | 226篇 |
基础医学 | 1680篇 |
口腔科学 | 258篇 |
临床医学 | 2200篇 |
内科学 | 2375篇 |
皮肤病学 | 221篇 |
神经病学 | 707篇 |
特种医学 | 728篇 |
外国民族医学 | 3篇 |
外科学 | 1593篇 |
综合类 | 3127篇 |
现状与发展 | 7篇 |
一般理论 | 2篇 |
预防医学 | 1279篇 |
眼科学 | 333篇 |
药学 | 1842篇 |
23篇 | |
中国医学 | 1163篇 |
肿瘤学 | 1213篇 |
出版年
2024年 | 26篇 |
2023年 | 269篇 |
2022年 | 477篇 |
2021年 | 836篇 |
2020年 | 727篇 |
2019年 | 599篇 |
2018年 | 592篇 |
2017年 | 616篇 |
2016年 | 576篇 |
2015年 | 807篇 |
2014年 | 971篇 |
2013年 | 1010篇 |
2012年 | 1373篇 |
2011年 | 1484篇 |
2010年 | 1006篇 |
2009年 | 879篇 |
2008年 | 960篇 |
2007年 | 889篇 |
2006年 | 788篇 |
2005年 | 673篇 |
2004年 | 523篇 |
2003年 | 571篇 |
2002年 | 435篇 |
2001年 | 369篇 |
2000年 | 284篇 |
1999年 | 264篇 |
1998年 | 191篇 |
1997年 | 208篇 |
1996年 | 163篇 |
1995年 | 139篇 |
1994年 | 132篇 |
1993年 | 105篇 |
1992年 | 62篇 |
1991年 | 58篇 |
1990年 | 51篇 |
1989年 | 78篇 |
1988年 | 52篇 |
1987年 | 53篇 |
1986年 | 27篇 |
1985年 | 38篇 |
1984年 | 22篇 |
1983年 | 27篇 |
1982年 | 21篇 |
1981年 | 19篇 |
1980年 | 23篇 |
1978年 | 6篇 |
1977年 | 10篇 |
1976年 | 5篇 |
1975年 | 8篇 |
1966年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 62 毫秒
82.
Dan-Dan Shan Qiu-Xian Zheng Jing Wang Zhi Chen 《World journal of gastroenterology : WJG》2022,28(16):1641-1655
Cancer has become the most life-threatening disease in the world. Mutations in and aberrant expression of genes encoding proteins and mutations in noncoding RNAs, especially long noncoding RNAs (lncRNAs), have significant effects in human cancers. LncRNAs have no protein-coding ability but function extensively in numerous physiological and pathological processes. Small nucleolar RNA host gene 3 (SNHG3) is a novel lncRNA and has been reported to be differentially expressed in various tumors, such as liver cancer, gastric cancer, and glioma. However, the interaction mechanisms for the regulation between SNHG3 and tumor progression are poorly understood. In this review, we summarize the results of SNHG3 studies in humans, animal models, and cells to underline the expression and role of SNHG3 in cancer. SNHG3 expression is upregulated in most tumors and is detrimental to patient prognosis. SNHG3 expression in lung adenocarcinoma remains controversial. Concurrently, SNHG3 affects oncogenes and tumor suppressor genes through various mechanisms, including competing endogenous RNA effects. A deeper understanding of the contribution of SNHG3 in clinical applications and tumor development may provide a new target for cancer diagnosis and treatment. 相似文献
83.
目的:观察右归饮对去卵巢血管钙化中谷氨酸受体变化的影响。方法:将40只远交群雌性大鼠(sprague dawley,SD)随机分为假手术组、模型组、低剂量组、高剂量组和辛伐他汀组,采用皮下注射华法令加维生素D3制备血管钙化模型,造模后,低剂量组、高剂量组分别给予右归饮浓缩液5.35 g/(kg·d)和16.05 g/(kg·d)灌胃,辛伐他汀组给予辛伐他汀混悬水溶液5.25 mg/(kg·d)灌胃,假手术组和模型组给予等量生理盐水,共12周;实验结束时采集血浆标本测定雌二醇及血脂四项,采用苏木精-伊红染色法(HE染色)观察动脉钙化的情况,并采用免疫印迹试验检测N-甲基-D-天门冬氨酸受体(NMDAR,NR)1蛋白的表达。结果 :模型组大鼠雌二醇、甘油三酯含量和NR1蛋白表达降低,P<0.05,低密度脂蛋白升高,P<0.05;高剂量组和低剂量组雌二醇、甘油三酯含量升高,NR1蛋白表达增加,P<0.05;高剂量组、低剂量组和辛伐他汀组低密度脂蛋白的含量降低,P<0.05;辛伐他汀组雌二醇无变化,P>0.05。结论:右归饮可抑制大鼠去卵巢血管钙化的形成,可能与提高雌激素水平和NR1蛋白的表达有关。 相似文献
84.
Sheng-Qiang Gao Li-Dong Huang Rui-Jie Dai Dong-Dong Chen Wei-Jian Hu Yun-Feng Shan 《International journal of clinical and experimental pathology》2015,8(11):14779-14785
Peripheral blood-derived inflammation-based scores such as the neutrophil-lymphocyte ratio (NLR) have recently been proposed as prognostic markers in ulcerative colitis. In some previous serological markers are commonly used to detect the severity of the Crohn’s disease (CD), but their sensitivity and specificity are relatively low. So we want to use simple indicators which are easy to obtain to predict disease severity. Now, we investigated and compared the capacity of NLR and other inflammatory markers in detecting CD activity and differentiating CD patients from healthy controls. These CD patients had not received corticosteroid or immunosuppressive drugs within a defined period of time. Data from our hospital between 2010 and 2012 was used. Neutrophil-lymphocyte ratio (NLR), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), white blood cells (WBC), platelet count and albumin were measured in 44 patients with active CD, 66 patients with inactive CD, and 55 healthy blood donors. Disease activity was assessed by the Crohn’s Disease Activity Index. In the active CD group, NLR values were found to be elevated compared to inactive CD patients and controls (6.00±7.38, 5.53±6.18 and 1.84±0.85, respectively), but statistical difference was not found between active and inactive CD groups. The overall accuracy of NLR (cutoff: 2.13 fl), CRP (cutoff: 10.5 mg/dl), ESR (cutoff: 19.5 mm/hour) and WBC (cutoff: 9.2 × 109/l) in differentiating CD patients from healthy controls was 80.9%, 67.3%, 71% and 60% respectively. NLR values were found to be correlated with WBC and CRP levels. NLR increased in CD patients compared with healthy subjects. NLR had the best accuracy in determination of CD patients and healthy controls. NLR did not show a discriminative value in disease activity. 相似文献
85.
Wen Da Guan Chris Ka Pun Mok Zi Lin Chen Li Qiang Feng Zheng Tu Li Ji Cheng Huang Chang Wen Ke Xilong Deng Yun Ling Shi Guan Wu Xue Feng Niu Ranawaka A Perera Yuan Da Xu Jincun Zhao Lin Qi Zhang Yi Min Li Rong Chang Chen Malik Peiris Ling Chen Nan Shan Zhong 《Emerging infectious diseases》2015,21(12):2278-2280
86.
改革开放以来,中国医疗保障行政管理职能和机构设置不断调整,形成了不同的医疗保障行政管理体制,改革朝着更加科学、更为规范的方向发展。但是,当前医疗保障行政管理体制还没有调整到位,主要是医疗保障制度由不同的部门分割管理,造成了重复建设、本位主义、社会不公、难以适应人口流动等诸多许多问题。下一步应在政府职能转变的前提下,统筹卫生服务和医疗保障职能,建立健康保障大部门体制。 相似文献
87.
Shan Bao Shu-Ying Yang Zhuo-Ri Li Ge-Bo Wen 《Asian Pacific journal of tropical medicine》2014,7(2):149-152
Objective:To screen,identify,and compare the serum biomarkers between anovulatory dysfunctional uterine bleeding(ADUB)and ovulatory dysfunctional uterine bleeding(ODUB)in Lizu females.Methods:The subjects included 128 ADLB patients,63 ODUB patients,and 93controls.The serum and supernate of the subjects'mense were collected and stored at-80°C until use.Differential proteins in the sera of three groups were screened using surface-enhaneed laser desorption ionization time-of-flight mass spectrometry.The screened proteins were then identified by tricine-SDS-PAGE gel and spectrometry.Protein expression levels in the menses of ADUB,ODUB,and control subjects were determined using ELISA,RT-PCR,and Western blotting.SPSS 14.1 was used for statistical analysis and chart drawing(a=0.05),Results:Three differentia)protein peaks with peak values of 11.80,13.59,and 14.68 km/z were,screened and identified as serum amyploid protein A(SAA),vascular endothelial growth factor,and vitamin K epoxide reductase,respectively.The SAA was highly expressed in the menses of ADUB and ODUB patients but poorly expressed in the controls.The vascular endothelial growth factor was highly expressed in the menses of ODUB and controls but poorly expressed in ADUB patients.Meanwhile,the vitamin K epoxide reductase was highly expressed in the menses of ADUB and control subjects but poorly expressed in ODUB patients.Conclusions:The SAA is the common serum biomarker of ADUB and ODUB.ADUB may be related to angiogenesis impairment,whereas ODUB may be associated with blood coagulation disruption. 相似文献
88.
Baicalein increases keratin 1 and 10 expression in HaCaT keratinocytes via TRPV4 receptor activation 下载免费PDF全文
In this study, we characterized the effect of baicalein on the regulation of keratinocyte differentiation and proliferation, which are abnormal in atopic dermatitis or psoriasis. Treatment of HaCaT keratinocytes with 10 μm baicalein slightly inhibited cell growth, caused morphological differentiation and increased expression of keratins 1 and 10 (K1/K10) without affecting ROS generation, cytochrome c release or apoptosis. Baicalein treatment caused growth arrest in G0/G1 phase and also induced Ca2+ influx via TRPV4 receptor activation. Phosphorylation of ERK, Akt and p38 MAPK, but not JNK, was increased by baicalein, and inhibition of phosphorylation of ERK, but not that of Akt or p38 MAPK, blocked the baicalein‐induced increase in K1/K10 expression, suggesting that ERK activation is involved in this increase. Removal of extracellular Ca2+ or blockade of Ca2+ influx by pharmacological inhibition or silencing of the TRPV4 receptor did not affect growth arrest, ROS generation or apoptosis, but inhibited baicalein‐induced ERK phosphorylation and K1/K10 expression. Thus, baicalein treatment increases differentiation, and decreases proliferation, of keratinocytes. The mechanism of differentiation of keratinocytes is distinct from that of proliferation, the former being Ca2+ dependent and the latter Ca2+ independent. 相似文献
89.
目的探讨肝移植术后缺血性胆道病变(ITBL)患者接受介入治疗的安全性及疗效。方法回顾性分析2006年1月至2014年2月在中山大学附属第三医院介入血管科接受介入治疗的76例肝移植术后ITBL患者的影像及临床资料。按胆道造影表现分为3组:肝门区狭窄组(28例),肝内多发狭窄组(42例),胆汁瘤组(6例)。治疗方式包括单纯经皮经肝胆管引流术(PTBD)、PTBD配合球囊扩张、PTBD配合球囊扩张及胆道内支架术。引流方式包括胆道外引流及胆道内外引流。术后随访并观察疗效及胆道并发症。结果 76例患者的PTBD首次成功率为97%(74/76)。介入治疗的总体治愈率、好转率及无效率分别为21%(16/76)、51%(39/76)、28%(21/76)。其中,肝门区狭窄组治愈10例(36%),好转16例(57%),无效2例(7%),治疗有效22例(93%);多发性狭窄组治愈6例(14%),好转21例(50%),无效15例(36%),治疗有效27例(64%);胆汁瘤组好转2例(2/6),无效4例(4/6)。肝门区狭窄患者的疗效优于多发性狭窄患者(P0.05),多发性狭窄患者的疗效优于胆汁瘤患者(P0.001)。引流管期间主要并发症为胆道感染,其中外引流及内外引流胆道感染发生率分别为20%(13/64)及67%(8/12),比较差异有统计学意义(P0.001)。结论 PTBD是治疗肝移植术后ITBL的安全、有效手段之一。结合球囊扩张及内支架置入可有效改善患者症状,提高生存质量。采用胆道外引流可显著降低胆道感染发生率。 相似文献
90.
Laparoscopic rectopexy for recurrent volvulus of J pouch after total proctocolectomy and ileal pouch anal anastomosis 下载免费PDF全文
Suet‐Ying Lee Ka‐Lung Fok Hester Yui‐Shan Cheung Chung‐Ngai Tang 《Surgical Practice》2015,19(3):133-136
Ileal‐pouch anal anastomosis (IPAA) is most commonly performed after total proctocolectomy for ulcerative colitis and familial adenomatous polyposis. Small‐bowel obstruction is one of the complications that can occur years after the operation, but intestinal obstruction due to volvulus of the J pouch is a rare condition. We report a case of recurrent volvulus in a patient who had undergone IPAA after total proctocolectomy for cancer of the rectum and multiple colonic polyps. The patient underwent laparoscopic rectopexy and had no recurrence of volvulus. 相似文献